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. 2018 Apr 11;8:5805. doi: 10.1038/s41598-018-24046-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confirmation of neurofibromin knockdown by Western blot (A) and quantitative RT-PCR (B) in four cell lines used in the study. All cell lines showed an increase in phosphorylated ERK protein by Western blot (A). (C) Quantitation of the increased phospho-ERK signal did not achieve statistical significance (values are mean +/− standard deviation of 3 independent replicates for NHA, T98G, and SF268, and of 2 replicates for SF295; in a third SF295 assay, P-Erk levels were too low for reliable quantitation).