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. 2018 Apr 11;8:5838. doi: 10.1038/s41598-018-24196-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FISH localization of centromeric satellites. Three satellites located in the centromere of chromosome 1 (VfSat6, VfSat7 and VfSat10) are shown separately in panels a–c. Centromeres of four other chromosomes that each contain a single satellite repeat are labeled in panels d–g: (d) VfSat13 repeat located in centromere 5, (e) VfSat8 in centromere 4, (f) VfSat16 in centromere 6, and (g) VfSat23 in centromere 2. Arrow in (a) points to minor non-centromeric locus of VfSat6 which is detectable only on a fraction of chromosomes. Hybridization patterns of FokI repeats (green signals) were used for chromosome discrimination in (e–g). Chromosomes counterstained with DAPI are shown in gray.