Table 1.
Satellite repeats investigated in this study. Novel satellite repeats are numbered with the prefix “VfSat”; references to previously described repeats are given in Notes. Names in square brackets refer to homologous repeats that were partially characterized by Novák et al.28. The column “ChIP-seq” provides ChIP/input ratios; the values of significant enrichment are highlighted and supplemented with repeat localization determined by FISH. Sequences of all newly identified satellites are provided in Supplementary Data S10.
| Satellite | monomer [bp] | Genomic abundance | Sequence characteristics | ChIP-seq (centromere) | Notes | ||||
|---|---|---|---|---|---|---|---|---|---|
| [%] | [Mbp/1 C] | % AT | max A/T | max C/G | max Pu/Py | ||||
| VfSat1 | 191 | 2.723 | 365.1 | 75.9 | 1.07 | 1.88 | 1.22 | 1.1 | [Vf_TA_11]; similarity to TR-9 from P. sativum |
| FokI | 59 / 57 | 2.322 | 311.4 | 59.3 | 1.44 | 2.00 | 1.11 | 1.0 | 37 |
| VfSat2 | ~26 | 1.292 | 173.2 | 71.4 | 2.60 | 5.00 | 2.00 | 0.4 | |
| VfSat3 | 702 | 0.329 | 44.1 | 68.1 | 1.24 | 1.13 | 1.21 | 0.3 | [Vf_TA_39] |
| TIII15 | 58 | 0.222 | 29.8 | 53.4 | 1.39 | 2.86 | 1.90 | 1.9 | 40 |
| VfSat4 | 38 | 0.199 | 26.7 | 73.7 | 1.80 | 2.33 | 1.92 | 0.6 | similarity to VicTR-B |
| VfSat5 | 687 | 0.187 | 25.1 | 77.6 | 1.16 | 1.19 | 1.07 | 0.3 | |
| pVf7 | 169 | 0.182 | 24.4 | 55 | 1.07 | 1.00 | 1.04 | 0.3 | 38 |
| VfSat6 | 50 | 0.132 | 17.6 | 64 | 1.91 | 1.57 | 1.78 | 103.6 (CEN1) | similarity to TR-5 from P. sativum |
| VfSat7 | 44 | 0.102 | 13.7 | 70.5 | 1.21 | 1.17 | 1.2 | 103.2 (CEN1) | |
| VfSat8 | 2033 | 0.061 | 8.1 | 71.7 | 1.68 | 1.03 | 1.46 | 91.3 (CEN4) | |
| VfSat9 | 963 | 0.055 | 7.4 | 77.6 | 1.02 | 1.77 | 1.00 | 0.2 | |
| VfSat10 | 1762 | 0.042 | 5.7 | 74.7 | 1.08 | 1.27 | 1.00 | 41.2 (CEN1) | |
| VfSat11 | 1619 | 0.040 | 5.3 | 75.7 | 1.08 | 1.23 | 1.01 | 0.3 | |
| VfSat12 | 1004 | 0.038 | 5.1 | 74.2 | 1.12 | 1.27 | 1.02 | 0.2 | |
| VfSat13 | 47 | 0.036 | 4.8 | 68.1 | 2.56 | 1.14 | 1.76 | 149.2 (CEN5) | |
| VfSat14 | 888 | 0.035 | 4.7 | 75.6 | 1.06 | 1.33 | 1.02 | 0.3 | |
| VfSat15 | 942 | 0.035 | 4.7 | 76.5 | 1.11 | 1.03 | 1.08 | 0.3 | similarity to TR-20 from P. sativum |
| VfSat16 | 1712 | 0.038 | 5.1 | 65.1 | 1.20 | 1.00 | 1.27 | 109.9 (CEN6) | [Vf_TA_157] |
| VfSat17 | 781 | 0.031 | 4.2 | 75 | 1.11 | 1.10 | 1.06 | 0.4 | |
| VfSat18 | 1172 | 0.031 | 4.2 | 79.7 | 1.11 | 1.03 | 1.08 | 0.3 | |
| VfSat19 | 1345 | 0.024 | 3.3 | 80.2 | 1.01 | 1.35 | 1.07 | 0.2 | |
| VfSat20 | 924 | 0.022 | 3 | 74.5 | 1.23 | 1.29 | 1.24 | 0.7 | |
| VfSat21 | 1057 | 0.017 | 2.3 | 74.3 | 1.02 | 1.35 | 1.10 | 0.2 | |
| VfSat22 | 1834 | 0.016 | 2.2 | 71.8 | 1.25 | 1.22 | 1.11 | 0.3 | |
| VfSat23 | 1325 | 0.008 | 1.1 | 73.3 | 1.17 | 1.41 | 1.18 | 81.9 (CEN2) | |