Figure 1.

HIF3α expression in hMSCs. (a) Immunofluorescence analysis of HIF3α protein in hMSCs cultured in standard oxygen conditions (Normoxia), and in CoCl₂-induced hypoxia (Hypoxia) for 24 h in absence and in presence of indicated cytokines and probed with antibodies against HIF3α. Scale bars: 10 μm). (b) Immunofluorescence analysis of HIF3α protein in cells grown in normoxia and hypoxia with siRNA-mediated HIF3α silencing or scrambled siRNA as control (scr). (c) Expression levels of the three alternative first exons (1a, 1b, and 1c) by qRT-PCR in hMSCs cultured in normoxia or hypoxia for 24 h in absence or in presence of indicated cytokines. Relative gene expression data are reported as 2-ΔΔCt method, normalized to housekeeping gene (b-actin mRNA) and ALU sequences. Data are expressed as means ± SEM (n = 3).*p value < 0.05.