Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 11;8:5842. doi: 10.1038/s41598-018-24221-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Immunohistochemical analysis of Hif3α and Cd45 expression in uninjured rat carotids and in carotids from Wistar male rats treated with MSCs or DMEM and harvested 7 days after arteriotomy. (a) Uninjured rat carotid; (b) arteriotomy-injured rat carotid harvested 7 days after injury, haematoxylin staining. Arrows in b indicate the injury site, where arteriotomy is followed by the application of an 8.0 polypropylene stitch (light blue). (c–f) Representative immunohistochemical staining of Hif3α in uninjured rat carotid (c) and in injured carotids harvested 7 days after arteriotomy from DMEM- (d) and MSC-treated rats (e). (g–l) Representative immunohistochemical staining of Cd45 in adjacent cross-sections from uninjured rat carotid (g) and in injured carotids harvested 7 days after arteriotomy from DMEM- (h) and MSC-treated rats (i). (f,l) Immunohistochemical staining of serial cross-sections of rat carotids used in d and h without primary antibody as negative control. (a,b) 10x magnification; (c–l) 20x magnification; small insets: 40x magnification of selected areas enclosed in black rectangles, representative of nuclei positive to Hif3α and Cd45 in adventitia, vasa vasorum and perivascular tissue. Brown staining corresponds to target protein expression. Nuclei were counterstained with haematoxylin. L: lumen; M: media; A: adventitia.