Fig. 2.

Colony growth of TRCs was abrogated by WYC-209. a None: non-treated B16-F1 cells cultured in 90-Pa fibrin gels. DMSO: Control B16-F1 cells cultured in 90-Pa fibrin gels and treated with 0.1% DMSO (dimethyl sulfoxide, solvent for WYC-209) on day 0. WYC-209 0.001 μM, WYC-209 0.01 μM, WYC-209 0.1 μM, WYC-209 1 μM and WYC-209 10 μM: Control B16-F1 cells were cultured in 90-Pa fibrin gels treated with WYC-209 on day 0 with various concentrations. Tazarotene 10 μM: Control B16-F1 cells were cultured in 90-Pa fibrin gels treated with 10 μM Tazarotene on day 0 as positive control. P < 0.002 between DMSO and WYC-209 1 µM; P < 0.0004 between DMSO and WYC-209 10 µM on day 5; P < 1.1e⁻⁷ between WYC-209 10 µM and Tazarotene 10 μM on day 5. Mean ± s.e.m.; ^**P < 0.01; ^***P < 0.001; at least three independent experiments. b Representative images of colonies on day 5 in a; Scale bar, 50 μm. c Dose-effect curves of WYC-209, WYC-209A, and WYC-209B. Quantified colony sizes on day 5 after B16-F1 cells were cultured in 90-Pa fibrin gels and treated with different conditions. None: medium without drugs. DMSO: medium with 0.1% DMSO. Mean ± s.e.m.; three separate experiments. d IC₅₀ of WYC-209, WYC-209A, WYC-209B, and Tazarotene. Student’s t-test was used in all statistics