Fig. 4.

WYC-209 inhibits colony growth of five different human cancer lines. Human ovarian cancer A2780 (a), human lung cancer A549 (b), human breast cancer MCF-7 (c), human melanoma MDA-MB-435s (d), and human malignant melanoma A375 (e) were cultured in 90-Pa fibrin gels for 3 days and treated with 0.1, 1, or 10 μM WYC-209. f–j A2780, A549, MCF-7, MDA-MB-435s, and A375 were cultured in 90-Pa fibrin gels for 3 days, treated with 10 μM WYC-209, and were then washed out of the compound on day 4. None: cell medium only, DMSO: medium with 0.1% DMSO. Note that because the cell doubling time was different for different types of cancer cells, the colony sizes varied. Mean ± s.e.m.; n = 15 samples; three separate experiments; ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. Student’s t-test was used in all statistics