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. 2018 Apr 11;8:5848. doi: 10.1038/s41598-018-24165-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Outflow facility (C) and (B) TM stiffness for two mouse strains. For each box, the central line represents the median, and the edges of the box are the 25th and 75th percentiles, and whiskers extend to the most extreme data points not considered outliers. Each dot represents the data from one eye per mouse, as described in more detail in the Statistical Analysis section. Due to technical issues, not all measurements in all mice were successful; number of eyes shown for facility plot are n = 12 for C57BL/6 J and n = 10 for CBA/J. For stiffness plot, the respective values are n = 18 and n = 10. NS: Not Significant. (C) Cross-plot between outflow resistance (1/C) and TM stiffness, with each data point representing one mouse. The black solid line and equation represent the linear regression of the pooled data. The gray-shaded region shows 95% confidence bounds for the regression. For each mouse, only the data from the OD eye was used, except in cases where the OD eye yielded invalid facility data due to technical issues, in which case data from the OS eye was used. Only mice where both outflow resistance and TM stiffness were measured in the same eyes were included. Number of data points: n = 12 for C57BL/6 J, n = 8 for CBA/J.