Figure 10.

Schematic diagram of the cryosection-based AFM technique. Sagittal cryosections were cut from a frozen eye and mounted to an adhesive glass slide without glue. The bottom figure shows the limbal region of a representative cryosection observed from the AFM bottom camera (SC = Schlemm’s canal). Numbers within the TM region indicate individual locations indented by the AFM probe. Scale bar: 50 µm. Section thickness: 10 µm.