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. 2018 Apr 11;8:5794. doi: 10.1038/s41598-018-24127-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flowchart of experimental design. Male Spargue Dawley (SD) rats were subjected to 90 min of ischaemia and finally euthanized. During the 90 min of ischaemia, rats were kept under general anaesthesia with isoflurane and buprenorphine. To achieve ischaemia, both femoral artery and vein were ligated with 4-0 silk. In addition, the right internal jugular vein was cannulated and used for the administration of the investigated drugs. For further details see the material and methods section.