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. 2018 Apr 11;9:1391. doi: 10.1038/s41467-018-03772-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Analytical performance of the Trioplex assay in multiplex format for all target viruses. Normal human serum was spiked with Zika virus, dengue virus type 1 (DENV-1), dengue virus type 2 (DENV-2), dengue virus type 3 (DENV-3) or dengue virus type 4 (DENV-4). Six serial dilutions (1:10) were tested to compare ZIKV detection performance of the Trioplex assay with the CDC ZIKV reference assay¹⁸ (a) or DENV detection with the CDC DENV reference assay²⁹ (b). Data for DENV-4 is shown. Straight line represents linear regression of CDC reference test. Dashed line represents linear regression for Trioplex assay. Trioplex assay limit of detection evaluated for each DENV serotype and chikungunya virus testing 20 replicates per dilutions: 1:100 before LoD (BLoD), 1:10 before LoD (BLoD), LoD and 1:10 after LoD (ALoD) (c). Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA are displayed at each dilution. Error bars represent GCE/mL standard deviation