Table 2.
Performance of Trioplex with archived clinical serum specimens
| Specimen category | Tested | ZIKVa positive | DENVa positive | CHIKVa positive | Positive percent agreement | Negative percent agreement |
|---|---|---|---|---|---|---|
| Zika | 20 | 19/20b | 0/20 | 0/20 | 95% (19/20) (76.4–99.1%) | 99.2% (134/135) (95.9–99.9%) |
| Dengue | 82 | 0/82 | 82/82 | 0/82 | 100% (82/82) (95.5–100%) | 100% (73/73) (95–100%) |
| Chikungunya | 13 | 0/13 | 0/13 | 13/13 | 100% (13/13) (77.2–100%) | 100% (142/142) (97.4–100%) |
| Negative | 40 | 1/40b | 0/40 | 0/40 | N/A | N/A |
N/A not applicable
a Trioplex component result
b One Zika specimen tested negative with the Trioplex assay but tested positive (CT 34.4) with the CDC Zika NS3 assay18. This specimen also tested positive for Zika IgM with the CDC Zika IgM Mac ELIZA