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. 2018 Apr 11;8:5851. doi: 10.1038/s41598-018-24206-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular cloning of HECI precursor cDNA and sequence alignments. (A) The nucleotide and translated open reading frame amino acid sequence of the cDNA encoding the biosynthetic precursor. The putative signal peptide is single underlined and the mature peptide is double underlined. (B) The alignment of prepropeptides of amphibian BBI peptides. (C) The alignment of prepropeptide encoding cDNAs of amphibian BBI peptides. (D) The alignment of amphibian skin-derived BBI mature peptides with the reactive site loop of peptides in bold typeface. Identical amino acids and nucleotides are indicated by asterisks.