Fig. 2.

β-Actin inhibits reprogramming and Srf activity. a Knockdown of Actb gene expression enhances reprogramming. Reprogramming efficiencies from NPCs are shown as iPSC colony numbers relative to shLuc + mock-introduced cells. Means ± SD are shown (n = 3). Dots indicate individual data points. Student’s t-test (*P < 0.05). b Knockdown of Actb alters various gene expressions. A scatter plot comparing gene expressions of Actb-knockdown NPCs and control cells. Blue dots and magenta dots show genes upregulated and downregulated > 2-fold, respectively. Some known Srf target gene symbols are indicated. c Srf-binding consensus motifs are enriched upstream of genes upregulated by Actb knockdown. Genes upregulated > 2-fold in NPCs were subjected to a motif search using Transfac to search protein-binding consensus sequences that were enriched in upstream regions. d Srf is activated by Actb knockdown. Expression levels relative to control cells for each Srf target gene in NPCs are shown (n = 2, technical duplicate). Values are means ± SD of the microarray data. Dots indicate individual data points