Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 11;9:1387. doi: 10.1038/s41467-018-03748-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Srf and its cofactor, Mkl1, promote reprogramming. a Srf promotes reprogramming. Values are means ± SD of the numbers of iPSC colonies produced relative to control cells (n = 3). OE indicates overexpression. Dots indicate individual data points. Student’s t-test (***P < 0.0005, **P < 0.005, *P < 0.05). b Mkl1 mainly localizes in the nucleus upon β-actin depletion. Immunofluorescence microscopy of NPCs using anti-Mkl1 antibody and images of the corresponding bright field and DAPI staining. Bar, 20 µm. c Mkl1 promotes reprogramming. Values are means ± SD of numbers of iPSC colonies generated from NPCs relative to the control (n = 3). Dots indicate individual data points. Student’s t-test (***P < 0.0005). d Srf promotes reprogramming at early phase. NPCs were infected with egfp-expressing lentivirus or Srf-overexpressing lentivirus at the days indicated (d1 is the day of reprogramming initiation by Dox addition). Vertical axis shows the reprogramming efficiency of Srf-overexpressing cells to egfp-expressing cells in logarithmic (log) scale (n = 3). Dots indicate individual data points. Asterisks indicate statistically significant differences between Srf overexpression and control on the day of virus infection (Student’s t-test; *P < 0.05)