Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 11;9:1387. doi: 10.1038/s41467-018-03748-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Srf preferentially downregulates cell-type-specific genes. a A scatter plot showing gene expressions that are > 2-fold different between Srf-overexpressing NPCs and control cells. Blue dots show NPC genes, which are defined as genes whose expression levels in NPCs are > 2-fold compared with those in ESCs. b Cell-type-specific genes are enriched in genes downregulated by Srf overexpression. Asterisks indicate a significant difference between genes in the indicated group and all other genes (two-sided Fisher’s exact test; ***P < 0.0005). c Srf preferentially binds to open and H3K27ac-marked genomic regions. The peak number in the overlapped area is based on the number of Srf-binding peaks. Overlaps were analyzed by the hypergeometric test. d Srf removes adjacent H3K27ac in NPCs. Means of ChIP-seq signals around genomic regions to which Srf was bound within 2000 bp from TSS (left panels) and other regions (right panel). e Matrices of consensus binding motifs of HDACs and Ep300 are enriched near Srf-binding sites. Motif analyses of ChIP-seq data of Srf in NPCs indicated that matrices of the motifs of HDAC1, HDAC2, and Ep300 were enriched within 25 bp of the Srf-binding sites in NPCs. Fisher’s exact test