Fig. 6.

Srf misactivation causes various diseases. a Srf activity is regulated by an exogenous cue, extracellular matrix stiffness. The expressions of Srf target genes in NPCs cultured on matrices of various stiffness relative to the value of each gene on the softest matrix (0.5 kPa). Values are means ± SD of the relative expression levels normalized to Gapdh expression (n = 3). Dots indicate individual data points. b Cell-type-specific genes are downregulated by Srf with stiffness. Percentages of genes differentially regulated in NPCs among cell-type-specific genes and other genes between 0.5 and 12 kPa matrices. Two-sided Fisher’s exact test (***P < 0.0005, **P < 0.005, *P < 0.05). c Structure of transgenes in KH2-Srf mice. d Srf misactivation causes diseases. Immunohistological observations of chimeric mice that were treated with Dox for 7 days. Upper images are representative images of hematoxylin/eosin staining (HE) and lower images are roughly corresponding view fields of immunohistochemistry sections for Srf ectopic expression (mCherry) in colon and pancreas in a chimeric mouse overexpressing Srf. Right panels of each tissue are magnified views of the areas indicated by the squares in the respective left panels. Arrows indicate sites exhibiting crypt abscess. Bars, 200 µm