Table 2.

Summary of nucleotide variants described with targeted amplicon sequencing

Amplicon region (amplicon length)	Sampled infections	Sequenced amplicons	Total variant sites	Nonsyn. variant sites	Singleton variants	Common variantsa	Highly variant sitesb
CSP (288 nt)
Total	1687	3821	55	50	15	23	12
West Africa	935	2147	46	43	8	24	8
East Africa	752	1674	41	39	8	23	8
TRAP (318 nt)
Total	4209	8774	52	52	13	15	5
West Africa	2334	4925	38	38	7	16	5
East Africa	1875	3849	38	38	10	15	3
SERA2 (258 nt)
Total	4209	9007	67	67	19	12	16
West Africa	2334	5048	53	53	17	13	12
East Africa	1875	3959	49	49	11	8	11

^a Minor allele frequency >0.02

^b >2 segregating alleles