Fig. 1. Schematic representations of the GOLD chip and the detection of allergen-specific IgEs. (A) Allergens are spotted onto hydrophilic polymer-coated glass slides in triplicate for immobilization. The assay is performed in a silicon chamber and the results are analyzed by fluorescence scanning. (B) Schematic representation of the array. The circles represent the spotting positions of the allergen extracts (in triplicate). (C) The allergens are immobilized via covalent bond formation between the NHS ester on the activated slide and an amine on the protein surface.
