Table I.
Fatty acid content and composition of fatty acids in transformed tobacco (SSU/ACL) leaves compared with those in control leaves
| Plant | Total Fatty Acid | Fatty Acid
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 16:0 | 16:1 | 16:2 | 16:3 | 18:0 | 18:1 | 18:2 | 18:3 | 22:1 | ||
| mg/g fresh wt | relative % (w/w) | |||||||||
| Control | ||||||||||
| 1 | 25.2 | 24.7 | 9.6 | 3.6 | 4.3 | 3.2 | 28.5 | 3.1 | 14.9 | 8.1 |
| 2 | 24.3 | 23.4 | 10.1 | 3.7 | 4.5 | 3.8 | 27.8 | 3.6 | 14.7 | 8.3 |
| SSU/ACL | ||||||||||
| T-2a | 24.8 | 24.1 | 8.9 | 3.5 | 3.7 | 3.6 | 29.5 | 3.8 | 14.2 | 8.7 |
| T-3 | 27.2 | 25.3 | 7.4 | 3.7 | 4.4 | 3.8 | 31.7 | 3.7 | 14.7 | 5.3 |
| T-4 | 27.4 | 25.9 | 7.5 | 3.8 | 4.4 | 3.2 | 31.6 | 3.1 | 15.6 | 4.9 |
| T-5 | 29.3 | 26.3 | 7.4 | 3.7 | 4.1 | 2.8 | 35.3 | 2.7 | 13.5 | 4.2 |
| T-6 | 28.3 | 29.4 | 5.5 | 2.8 | 2.7 | 2.2 | 35.1 | 2.3 | 15.8 | 4.2 |
| Control mean | 24.7 | 24.0 | 9.8 | 3.7 | 4.4 | 3.5 | 28.2 | 3.4 | 14.8 | 8.2 |
| SSU/ACL meanb | 28.7 | 26.7 | 6.9 | 3.5 | 3.9 | 3.0 | 33.4 | 3.0 | 14.9 | 4.7 |
Each analysis of the transformed tobacco was carried out in triplicate (on three separate leaves) with less than 3% variation in the proportions of individual fatty acids; the controls were assayed twice using six different leaves per assay, also with less than 3% variation.
a
This transformant showed only a meager increase in ACL activity over the control (see Fig. 3B), and its fatty acid profile is correspondingly closer to the control plants than the transformants.
b
Excluding T-2.