Table 2.
Summary of the SNPs included in the analysis.
| Polymorphism/position | Primer | Sequence (5′-3′) | TA (°C) | Product size (base pairs) | Restriction enzyme | Product size (base pairs) after digestion |
|---|---|---|---|---|---|---|
| DOG SNPs | ||||||
| −213AG/5′ flanking region | Forward Reverse |
CCA TTG GAA TCC GCC CCC T CAC CAC CAG GTC GGC TAT G |
56 56 |
635 | Hpy99I | C allele: 180+ 201 +254 G allele: 41 +160+180+254 |
| −94TC/5′ flanking region | Forward | CCA TTG GAA TCC GCC CCC T | 60 | 635 | ||
| Reverse | CAC CAC CAG GTC GGC TAT G | 60 | ||||
| C allele specific | CCG ATC TGC TGG TCC CGG | 60 | 295 | |||
| T allele specific | CCG ATC TGC TGG TCC CGA | 60 | ||||
| −74CG/5′ flanking region | Forward | CCA TTG GAA TCC GCC CCC T | 56 | 635 | BsiEI | C allele: 180+201+254 G allele: 41+160+180+254 |
| Reverse | CAC CAC CAG GTC GGC TAT G | 56 | ||||
| OWNER SNPs | ||||||
| rs53576/intron 3 | Forward | ACT GGG GCA ACC AAA CAT CT | 56 | 304 | AvaII | G allele: 133 + 61 + 110 A allele: 194 + 110 |
| Reverse | ACT CTT CAT GGC CCA GAG TG | 56 | ||||
| rs2254298/intron 3 | Forward | CTG TCT TTG CAC CTT TGC TA | 56 | 347 | DdeI | C allele: 276 + 71 T allele: 246 + 30 + 71 |
| Reverse | ATG AAA GCA GAG GTT GTG TG | 56 | ||||
| rs1042778/exon 4 | Forward | GCT CCA GCC AGA GGA G | 60 | 283 | ||
| Reverse | AGT GGG TTC AGG GTG GTA | 60 | ||||
| A allele specific | AGC CAC CCC AAG GAG T | 60 | 182 | |||
| C allele specific | AGC CAC CCC AAG GAG G | 60 | ||||