Fig. 4.
Response of GCaMP6s-targeted PVN CRH cells in vivo and results from performance comparison with a commercial system. (a) Experimental protocol following viral injection of the GCaMP6s vector, for which (b) representative traces from a GCaMP6s (green) and EYFP (orange) CRH-Cre mouse. Red bars denote transfer to or from the footshock cage that provides the conditioned stimulus. (c) Photon transfer curves for the two systems compared in this study were generated over a range of excitation powers appropriate to each system. Numbers indicate (power in uW, SNR). (d) Recording from a single GCaMP6s mouse using both systems to capture different segments of a continuous experiment. (Red bars denote transfer to or from a new environment.) (e), (f) detailed traces at transition times. Recordings made by the Doric solution at (orange, g) and CICLoPS at 100 nW (blue, h) during delivery of a series of footshocks. (i), (j) detailed traces during the footshock-delivery interval; black bars denote shock times (shock duration not to scale). CICLoPS offers similar performance as Doric at much lower excitation power leading to much lower photobleaching.