Figure 6.

Viability of untransformed BY2 (WT) cells and transgenic BY2 cells after hyperosmotic shock treatment. Five-day-old BY2 cells were treated with 180 mm mannitol (200 mOsm; A), 620 mm mannitol (700 mOsm; B), or 500 mm NaCl (900 mOsm; C) at a cell density of 50% (v/v) for 20 min, and then stained with FDA as described in “Materials and Methods.” Viable cells with normal membrane permeability were detected in fluorescence micrographs (right image) by comparison with corresponding phase-contrast micrographs of the cells (left image). Each value indicates the percentage of cell survival quantified from the photographs.