Figure 7.

In vivo analysis of astrocyte differentiation in HtrA1-deleted mice reveal increase in astrogliogenesis at P1. A–B′, Representative IF staining of Aldh1L1 (green) and EdU (magenta) in P1 brains of HtrA1^+/+ (n = 3) and HtrA1^−/− (n = 5) mice. Areas of analysis include the dorsomedial neocortex (A, A′) and the CA1 region of the hippocampal primordium (B, B′; dotted line demarcates CA1 and dentate gyrus). Aldh1L1⁺ cells (arrows) and Aldh1L1⁺EdU⁺ precursors (arrowheads) are both significantly increased in both regions in HtrA1^−/− mice. C, Quantification of Aldh1L1⁺ cells in the neocortex and the CA1 region of the hippocampus at P1. D, Quantification of Aldh1L1⁺EdU⁺ in the neocortex and the CA1 region of the hippocampus. A significant increase in both dividing and nondividing Aldh1L1⁺ cells were detected in HtrA1^−/− mice. E–F′, Images of the neocortex (E, E′) and hippocampus (F, F′) stained with Aldh1L1 (green) and EdU (magenta) at P7 in HtrA1^+/+ (n = 3) and HtrA1^−/− (n = 3) mice. G, H, Quantification of Aldh1L1⁺ and Aldh1L1⁺EdU⁺ cells in P7 neocortex and hippocampus. A reduction in Aldh1L1⁺EdU⁺ cells (arrowheads) in the neocortex of P7 HtrA1^−/− mice was observed. All data presented as mean ± SEM. Statistical significance was measured by unpaired Student's t test. Scale bar, 50 μm. *p < 0.05.