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. 2018 Mar 20;475(6):1075–1089. doi: 10.1042/BCJ20170418

Figure 2. Characterization of Sox/Oct motifs.

Figure 2.

(a) The top de novo sequence motifs (based on enrichment) detected by CisFinder [60] in Oct4 and Sox2 ChIP-Seq data from ESCs [49], shown by WebLogo. (b) Comparison of Nanog and Fgf4 Sox/Oct motifs across different eutherian species. A phylogenetic tree illustrated the sequence conservation of those Sox/Oct motifs in cumulative evolution. (c) Alignment of the Sox/Oct motifs from the target genes Nanog, Utf1, Oct4, Sox2, and Fgf4. (d) Mutations generated in the Sox/Oct motif in a 400 bp gene fragment from Nanog located at −289 to +117 (relative to the Nanog transcription start site) [31,61] were subsequently tested. (e) Luciferase activity of constructs shown in (d) after transfection of F9 embryonal carcinoma cells. R = 3, mean ± SD.