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. 2018 Apr 11;18:130. doi: 10.1186/s12906-018-2185-x

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Flow cytometric analysis of apoptosis induction in Jurkat (a, b), MCF7 (c, d) and Vero (e, f) cells. Cells were treated with various concentrations of water and ethanolic extracts prepared from the powdered formula of H. cordata fermented broth and P. emblica fruit for 24 h. The Annexin V-FITC/PI staining apoptotic cells were analyzed using flow cytometry. Actinomycin D (Act D; 10 μg/mL) was used as a positive control. Bar graph shows the summarized data from three independent experiments performed in duplicate compared with untreated control (c)