Table 1.
Osteogenesis and oncostatin M (OSM) pathway-related gene primer pairs in rat bone marrow mesenchymal stem cells used in quantitative reverse transcription-polymerase chain reaction
| Gene | Primer sequences |
|---|---|
| ALP | Forward: 5′-TCACTTCCGCCCGGAACCCT-3 |
| Reverse: 5′-TGTCCTGCCGGCCCAAGAGA-3′ | |
| RUNX2 | Forward: 5′-GCGGACGAGGCAAGAGTT-3′ |
| Reverse: 5′-TTGGTGCTGAGTTCAGGGAG-3′ | |
| OCN | Forward: 5′-TGAGGACCCTCTCTCTGCTC-3′ |
| Reverse: 5′-GGGCTCCAAGTCCATTGTT-3′ | |
| OPN | Forward: 5′-ATCTGAGTCCTTCACTG-3′ |
| Reverse: 5′-GGGATACTGTTCATCAGAAA-3′ | |
| BSP | Forward: 5′-ATAGGCAACGAGTACAACAC-3′ |
| Reverse: 5′-GTATCCAGATGCAAAGACAG-3′ | |
| COL-1 | Forward: 5′-TGTTCGTGGTTCTCAGGGTAG-3′ |
| Reverse: 5′-TTGTCGTAGCAGGGTTCTTTC-3′ | |
| OSMR | Forward: 5′-TGCGGACAGAGAATGGAAGA-3′ |
| Reverse: 5′-GACTCCGTTGGATTGGCTTC -3′ | |
| IL6st | Forward: 5′-TCCACATGGCATACACA −3′ |
| Reverse: 5′-GCTAAGCACACAGGCACGAC -3′ | |
| STAT3 | Forward: 5′-AGTCACACGCCACTCTGGTG −3′ |
| Reverse: 5′-GCTAAGCACACAGGCACGAC -3′ | |
| GAPDH | Forward: 5′-TGCACCACCAACTGCTTAGC-3′ |
| Reverse: 5′-GGCATGGACTGTGGTCATGAG-3′ |