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. 2018 Apr 11;11:236. doi: 10.1186/s13071-018-2823-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Schematic diagram of the polytetrafluoroethylene (PTFE) membrane-based blood-feeding system. a EDTA tube with blood. b 180 ml Styrofoam cup. c PTFE roll. d Prepared PTFE membrane-based blood meal on the underside of the Styrofoam cup. e Warm water necessary to achieve 37 °C blood meal. f Wooden holding board for holding the inverted cups with PTFE-covered blood meal. g Mosquito cage measuring 30 × 30 × 30 cm