Figure 1.

ILC1s Lack TRAIL Expression in NKp46-Deficient Mice

(A) Representative flow cytometry plots showing frequencies of T cells (CD3⁺ NK1.1⁻), NKT cells (CD3⁺ NK1.1⁺), and NK cells (CD3⁻ NK1.1⁺) in the livers of naive wild-type mice, Ncr1^−/− mice, or heterozygous Ncr1^+/− mice.

(B and C) Representative flow cytometry histograms (B) and average percentage (± SD) (C) of TRAIL⁺ group1 ILCs detected in the livers of Ncr1^+/− and Ncr1^−/− mice.

(D and E) Representative flow cytometry plots of TRAIL, CD49b/DX5, and CD49a expression on hepatic group 1 innate lymphoid cells (CD3⁻ NK1.1⁺) from naive Ncr1^+/− and Ncr1^−/− mice (D) and average percentage (± SD) of CD49b/DX5⁺ NK cells (E, left) and CD49a⁺ NK cells (E, right) as described in (D).

(F) Representative flow cytometry plots of the gating strategy used to distinguish (CD3⁻ NK1.1⁺) ILC subsets: mature NK cells (CD49b⁺Eomes⁺) from immature NK cells (CD49b⁺Eomes⁻) and ILC1s (CD49b⁻ Eomes⁻) in liver, lymph node (LN), and spleen tissues harvested from Ncr1^+/− and Ncr1^−/− mice.

(G) Representative flow cytometry histograms of TRAIL expression on the cell subsets defined in (F).

Data are representative of 2–4 experiments, each with 2–5 mice per group. ^∗∗∗∗p < 0.0001 (unpaired t test).