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. 2018 Apr 5;70(1):150–164.e6. doi: 10.1016/j.molcel.2018.02.023

Figure 6.

Figure 6

ZUFSP Is a Putative DNA Replication and Repair Factor

(A) Immunoprecipitation of endogenous ZUFSP reveals distinct biological functions. DAVID analysis for Gene Ontology Biological Processes (GOBP) showing enrichment for the top hits.

(B) Subset of ZUFSP interaction map showing proteins involved in DNA repair, DNA replication, and negative regulation of DNA recombination.

(C) HeLa cells were transfected with YFP-ZUFSP, subjected to laser micro-irradiation, and imaged at the indicated time. Scale bar, 10 μm.

(D) HeLa cells were transfected with YFP-ZUFSP, pre-treated with DMSO or MG132 (3 hr), subjected to laser micro-irradiation, and imaged at the indicated time and then fixed and processed for immunostaining with the indicated antibodies. Scale bar, 10 μm.

(E) HeLa wild-type (WT) of ΔUBC13 cells were transfected with YFP-ZUFSP, subjected to laser micro-irradiation, and imaged at the indicated time and then fixed and processed for immunostaining with the indicated antibodies. Scale bar, 10 μm.

See also Figure S6.