REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-53BP1	Millipore	Cat# MAB3802, RRID:AB_11212586
Mouse anti-FLAG HRP	Sigma	Cat# A8592, RRID:AB_439702
Mouse anti-γH2AX (Ser139)	Biolegend	Cat# 613402, RRID:AB_315795
Mouse anti-Ub (FK2)	Enzo	Cat# BML-PW8810, RRID:AB_10541840
Rabbit anti-53BP1	Santa Cruz Biotechnology	Cat# sc-22760, RRID:AB_2256326
Rabbit anti-Cyclin A	Santa Cruz Biotechnology	Cat# sc-751, RRID:AB_631329
Rabbit anti-GFP	Abcam	Cat# ab290, RRID:AB_303395
Rabbit anti-γH2AX (Ser139)	Abcam	Cat# ab81299, RRID:AB_1640564
Rabbit anti-RFWD3	Bethyl	Cat# A301-397A, RRID:AB_961090
Rabbit anti-SMARCAL1	Bethyl	Cat# A301-616A, RRID:AB_1211349
Rabbit anti-Tubulin	Abcam	Cat# ab184970
Rabbit anti-UBC13	Cell Signaling Technology	Cat# 4919S, RRID:AB_2211168
Rabbit anti-Ub (K63-specific, Apu3)	Millipore	Cat# 05-1308, RRID:AB_1587580
Rabbit anti-Ub	Dako	Cat# Z0458, RRID:AB_2315524
Rabbit anti-UFM1	Abcam	Cat# ab109305, RRID:AB_10864675
Rabbit anti-ZUFSP	Sigma	Cat# HPA044426, RRID:AB_10960887
Rabbit anti-ZUFSP	Custom (Eurogentec)	N/A
Bacterial and Virus Strains
pLenti CMV/TO Hygro DEST	Addgene	Cat# 17291
pLenti CMV TetR Blast	Addgene	Cat# 17492
pENTR4-GFP-C1	Addgene	Cat# 17396
pDONR221	Invitrogen	Cat# 12536017
pDEST12.2	Invitrogen	Cat# 11808011
pYFP DEST	In house	N/A
pFLAG-CMV DEST	In house	N/A
Chemicals, Peptides, and Recombinant Proteins
MG132	Merck Millipore	Cat# 474790
Propargylamine	Sigma Aldrich	Cat# P50900-5G
Selenomethionine	Molecular Dimensions	Cat# MD12-503B
SureBeads Protein A Magnetic Beads	Bio-Rad	3161-4011
HaloLink Resin	Promega	G1915
Deposited Data
Mass spectrometry proteomics data	ProteomeXchange Consortium via PRIDE repository	http://proteomecentral.proteomexchange.org
ZUFSP Structure factor and coordinates files	RCSB-PDB	https://www.rcsb.org/
Microscopy images	Mendeley	https://data.mendeley.com/datasets/hs6fgdvdg3/draft?a=8e80899a-da80-4a34-a0c9-3d045f523f61
Gel images	Mendeley	https://data.mendeley.com/datasets/x7p9vk5cvt/draft?a=d198a3ad-9673-453c-b036-6045c75380e9
Experimental Models: Cell Lines
U2 O-S	ATCC	Cat# HTB-96, RRID:CVCL_0042
HeLa S3	ATCC	Cat# CCL-2.2, RRID:CVCL_0058
293	ATCC	Cat# CRL-1573, RRID:CVCL_0045
Jurkat	ATCC	Cat# TIB-152, RRID:CVCL_0367
Oligonucleotides
pDONR221-ZUFSP FWD: GGGGACAAGTTTGTAC AAAAAAGCAGGCTTCATGCTTTCCTGTAATATTTGTGG	This paper	N/A
pDONR221-ZUFSP REV: GGGGACCACTTTGTACAAGA AAGCTGGGTATCAAGGAATCTTCTCGGCTGT	This paper	N/A
ZUFSP siRNA-resistant (#2) FWD (i): CATATGTACAAATT ATCACATACTTCAGGAACATGTT	This paper	N/A
ZUFSP siRNA-resistant (#2) REV (i): AACATGTTCCTGAAG TATGTGATAATTTGTACATATG	This paper	N/A
ZUFSP siRNA-resistant (#2) FWD (ii): ACAAATTATCACATA TTGCAAGAACATGTTGACTTG	This paper	N/A
ZUFSP siRNA-resistant (#2) REV (ii): CAAGTCAACATGTTC TTGCAATATGTGATAATTTGT	This paper	N/A
pX459-UBC13 FWD: CACCGGGCGTTGCTCTCATCTGGTT	This paper	N/A
pX459-UBC13 REV: AAACGATGAGAGCAACGCCCGTTAC	This paper	N/A
siCTRL: (siGENOME Non-targeting siRNA pool #1)	Dharmacon	Cat# D-001206-13
siCTRL: GGGAUACCUAGACGUUCUA		N/A
siZUFSP (#1): AUAUGGAACUUCAGAUAAC	This study	N/A
siZUFSP (#2): UUACCAUAUUCUUCAGGAA	This study	N/A
siZUFSP (#3): GGUCACAGUCGAACUGUUA	This study	N/A
siZUFSP (#4): CAGUCGAACUGUUAUUGGA	Ambion	Cat# s48009
siZUFSP (#5): GGAAGACUGUGAUCAACCA	Ambion	Cat# s48010
siZUFSP (#6): GGAACUUCAGAUAACAAGA	Ambion	Cat# s48011
siZUFSP (#7, siGENOME SMARTpool):	Dharmacon	Cat# M-015894-00
Software and Algorithms
Spotfire	Tibco	https://spotfire.tibco.com RRID:SCR_008858
CellProfiler	Broad Institute	http://cellprofiler.org RRID:SCR_007358
MaxQuant v 1.6.0.13	Tyanova, S. et al., 2016	http://www.coxdocs.org/doku.php?id=maxquant:common:download_and_installation#downloadRRID:SCR_014485
Saint Express 3.6.1	Teo et al., 2014	https://sourceforge.net/projects/saint-apms/files/
Prism	Graphpad	https://www.graphpad.com/scientific-software/prism/ RRID:SCR_002798
XDS	Kabsch, 2010	http://xds.mpimf-heidelberg.mpg.de/
CCP4 interface version 7.0.048	Winn et al., 2011	http://www.ccp4.ac.uk/
AIMLESS	Evans and Murshudov, 2013	http://www.ccp4.ac.uk/html/aimless.html RRID:SCR_015747
MAD phasing mode of Auto-Rickshaw	Panjikar et al., 2005	http://www.embl-hamburg.de/Auto-Rickshaw/
COOT	Emsley et al., 2010	http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ RRID:SCR_014222
REFMAC5	Murshudov et al., 1997	http://www.ccp4.ac.uk/html/refmac5/description.html RRID:SCR_014225
PDB-REDO	Joosten et al., 2014	http://www.cmbi.ru.nl/pdb_redo/
PyMOL		https://pymol.org/2/ RRID:SCR_000305