Figure 1.

The 2^−ΔCq comparison of foxl2, cyp17a1, ar, dmrt1, sox9, and star genes between males and females in the gonads (a), anal fin (b), body kidney (c), liver (d), and white muscle (e). The arithmetic mean of gapdh and β-actin reference genes was used for normalization. The data points are represented by arithmetic means ± SD on a logarithmic scale in base two. The statistical significance of the difference in expression was tested with one-way ANOVA using Tukey correction (^∗∗p ≤ 0.01 and ^∗∗∗∗p ≤ 0.0001).