Table 1.
Primers for qPCR analysis.
| GenBank | Gene | PCR product length (bp) | Primer name | Primer sequence |
|---|---|---|---|---|
| KX420683 | ar—androgen receptor | 197 | ar-F | 5′-CKTGACTCCCCGAACAATCA-3′ |
| ar-R | 5′-AAGGTAGCACGCTGGAACTC-3′ | |||
|
| ||||
| KX420684 | dmrt1—doublesex and mab-3 related transcription factor 1 | 129 | dmrt1-F | 5′-CCACCCTGTTCCACTTCCAG-3′ |
| dmrt1-R | 5′-GAAGWGGATGGTGCTGTGCT-3′ | |||
|
| ||||
| KX420685 | sox9—sex-determining region Y-box 9 | 115 | sox9-F | 5′-AGGCCGATTCCYCTCACTCT-3′ |
| sox9-R | 5′-TGCAYGTCTGTTTTGGGAGT-3′ | |||
|
| ||||
| KX420686 | foxl2—forkhead box L2 | 120 | foxl2-F | 5′-GCCCACCTCGTACAATCCTT-3′ |
| foxl2-R | 5′-CTTAGCTGCTGAGGGTGGTG-3′ | |||
|
| ||||
| KX420678 | cyp17a1—cytochrome P450 family 17 subfamily A polypeptide 1 | 134 | cyp17a1-F | 5′-CCGTCGCTTACCTCCTACAC-3′ |
| cyp17a1-R | 5′-CCGTATCGTTGCTTCCAGGT-3′ | |||
|
| ||||
| KX420679 | star—steroidogenic acute regulatory protein | 111 | star-F | 5′-AGTACCCTGACCGCCTGTAT-3′ |
| star-R | 5′-TTGTGTCCTGCCCAATCCTC-3′ | |||
|
| ||||
| KX420681 | β-actin | 161 | β-actin-F | 5′-TGACCCTGAAGTAYCCMATC-3′ |
| β-actin-R | 5′-CTTCTCTCTGTTRGCYTTGG-3′ | |||
|
| ||||
| KX420682 | gapdh—glyceraldehyde 3-phosphate dehydrogenase | 114 | gapdh-F | 5′-AGACACCCGCTCNTCHATCT-3′ |
| gapdh-R | 5′-TCCACGACTCTGTTGCTGTA-3′ | |||
|
| ||||
| KX420680 | 28S rRNA—28S ribosomal RNA | 160 | 28S-F | 5′-TGTTTGTGAATGCAGCCCAA-3′ |
| 28S-R | 5′-GACCCCATCCGTTTACCTCT-3′ | |||