Figure 7.

Changes in Gene Expression Correlate with Specific Changes in DNA Methylation

Demethylation profiles of early (A), progressive (B), and late (C) upregulated genes across serial biopsies of CTVT-17 (red), CTVT-5 (yellow), and CTVT-6 (green). Demethylation scores were obtained for individual genes by quantifying demethylation levels within specific regions of genes (≤2 kb upstream of first exon; first exon; first intron; internal exons; internal introns; last exon; ≤2 kb downstream of last exon) and normalized by subtracting the corresponding demethylation values observed for non-expressed genes. Boxplots illustrate the variation within these values across each gene-list (boxes extend to the first and third quartile, whiskers extend to 1.5× inter-quartile range, and the line represents median values). For each CTVT sample, boxplots are in order (from left to right): first, second and third biopsy.

See also Figure S5.