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. 2018 Apr 9;33(4):736–751.e5. doi: 10.1016/j.ccell.2018.02.011

Figure 3.

Figure 3

High GKAP Expression Is Associated with Increased NMDAR Pathway Activity In Vivo

(A) qRT-PCR evaluation for the NMDAR subunits GluN1 (Grin1), GluN2b (Grin2b), and the scaffold protein GKAP (Dlgap1) in PanNET tumors from the two genetic backgrounds. Mean ± SEM. Mann-Whitney test was used to compare the expression of each gene in the B6 and C3H tumors. ∗p = 0.0175 (qRT-PCR: n = 7 tumors/7 mice/background).

(B) Western blot of GluN2b and GKAP in PanNETs from B6 and C3H backgrounds. After normalization, the one-column t test was used for comparison, hypothetical value = 1; ∗p = 0.01; n.s., not significant (mean ± SEM; n = 4 tumors/4 mice).

(C) Immunohistochemistry (IHC) analysis of large T oncoprotein, GluN2b, and p-GluN2b in PanNET tumor tissue sections. Images are representative of >50 tumors from >10 RIP1Tag2 mice/background. S, spleen; LN, lymph node.

(D) MK801 treatment in RIP1Tag2 mice. Cohorts of seven to nine mice were used for control (saline treated) and MK801 treatment in each genetic background; mean ± SEM. Mann-Whitney test: ∗∗p < 0.01; n.s., not significant.