Table 1.
The primers used for RT-qPCR analysis. ALP: alkaline phosphatase; Runx2: runt-related transcription factor 2; and Ocn: osteocalcin.
| Target gene | Primers | Sequence | Fragment size (bp) |
|---|---|---|---|
| ALP | Forward primer | 5′- ATAACGAGATGCCACCAGAGG-3′ | 140 bp |
| Reverse primer | 5′- TTCCACATCAGTTCTGTTCTTCG-3′ | ||
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| |||
| RUNX2 | Forward primer | 5′- CTACCCAGCCACCTTTACCTAC-3′ | 190 bp |
| Reverse primer | 5′- GAACTGATAGGATGCTGACGAAG-3′ | ||
|
| |||
| OCN | Forward primer | 5′- AGGAGGGCAATAAGGTAGTGAAC-3′ | 147 bp |
| Reverse primer | 5′- AGGCGGTCTTCAAGCCATAC-3′ | ||
|
| |||
| β-actin | Forward primer | 5′- GAGACCTTCAACACCCCAGC-3' | 263 bp |
| Reverse primer | 5′- ATGTCACGCACGATTTCCC-3′ | ||