Fig. 2.

The expression and purification of arginine decarboxylase from P. aeruginosa. E. coli strain TOP 10 harboring the speA gene within the pBAD cloning vector was induced with varying amounts of arabinose and the cell lysates were purified using the imparted 6-His tag and a nickel agarose column described in the methods. The eluted samples were analyzed by SDS-PAGE 10% acrylamide gels and stained with Coomassie dye. The eluate from the 2% arabinose condition was used for synthesis of isotopically labeled agmatine