Table I.
Purification of alliinase from A. cepa roots
| Fraction | Total Protein | Total Activity | Specific Activity | Purification | Recovery | |
|---|---|---|---|---|---|---|
| mg | nkat | nkat mg−1 | fold | % | ||
| Crude extract | 193.2 | 6,000 | 31 | 1.0 | 100 | |
| CM-Sepharose CL6B | 13.05 | 5,000 | 383.2 | 12.4 | 83 | |
| Con A-Sepharose, 5 mm methyl-d-glucopyranoside (I) | 0.243 | 513 | 2,109.8 | 68.1 | 8.6 | }57.5 |
| Con A-Sepharose, 200 mm methyl-d-mannopyranoside (II) | 0.940 | 2,937 | 3,124.2 | 100.8 | 48.9 | |
| CM-Sepharose CL6B, 5 mm methyl-d-glucopyranoside (I) | 0.062 | 210 | 3,380.7 | 109.0 | 3.5 | }28.8 |
| CM-Sepharose CL6B, 200 mm methyl-d-mannopyranoside (II) | 0.761 | 1,519 | 1,995.6 | 64.4 | 25.3 | |