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Effect of experimental diets on liver enzymes. HFFr: High-Fat, High-Fructose diet, S: Saline, +Met25: HFFr+25 mg/kg body weight metformin, +Met200: HFFr+200 mg/kg body weight metformin, +CaD: HFFr+2.4% Calcium plus 10000 IU/kg vitamin D3, ALT: Alanine aminotransferase, AST: Aspartate aminotransferase. Data are mean ± SE. n = 6–7 in each group. (*) P<0.05 versus the Control group. Values with different letters are significantly different (P<0.05) (one way ANOVA, Tukey post hoc test).
