Figure 4.
Western blotting analysis of AMPK and p-AMPK expression in the livers of experimental rats. Image shows demonstrative bands of the analyzed proteins in the livers. Bars represent the ratio of phosphorylated AMPKα to total AMPKα. HFFr: High-Fat, High-Fructose diet, S: Saline, +Met25: HFFr+25 mg/kg body weight metformin, +Met200: HFFr+200 mg/kg body weight metformin, +CaD: HFFr+2.4% Calcium plus 10000 IU/kg vitamin D3, AMPK: AMP-activated protein kinase. Data are mean ± SE. n = 6–7 in each group. There were no significant differences between groups (P>0.05).