Fig. 5. The siRNA silencing ability in the MEF and MEF Ago2^–/– cells. The Renilla mRNA reduction in the MEF and MEF Ago2^–/– cells. 1 nM of the unmodified (wt) and modified (OMe2, PS2, RIB2, ACR2, and THR2) siRNAs were co-transfected with a psiCHECK2 (AS) reporter. After 24 h, the cells were harvested for RNA extraction and qRT-PCR measurement [n = 3 ± SD]. The mock transfection, only the psicheck2 AS vector, was set as 1.