Table 1. Selected phosphosites observed in this study as being dependent on DIA1 presence and related to the secretory pathway or otherwise related to the predicted DIA1 kinase activity.
| Protein ID | Protein name | Gene name | Phosphosite motif | Phosphosite position | Student’s T-test p-value DIA_WT | Student’s T-test q-value DIA_WT | DIA1 vs. WT FC | Localisation |
|---|---|---|---|---|---|---|---|---|
| Q9H0W5 | Coiled-coil domain-containing protein 8 | CCDC8 | PQASPRR | 261 | NA | NA | UP | Cytoskeleton, cytoplasm |
| Q59H18 | Serine/threonine-protein kinase TNNI3K | TNNI3K | EVFTQCT | 637 | NA | NA | UP | Nucleus, cytoplasm |
| Q9H307 | Pinin | PNN | QQDSQPE | 381 | 0.0004 | 0.028 | 3.7 | Nucleus |
| Q9NR31 | GTP-binding protein SAR1a | SAR1A | IDRTDAI | 139 | 0.0018 | 0.045 | 2.1 | ER/Golgi |
| Q9H8Y8 | Golgi reassembly-stacking protein 2 | GORASP2 | MAGTPIT | 222 | 0.0014 | 0.038 | 2.0 | Golgi |
| Q66PJ3 | ADP-ribosylation factor-like protein 6-interacting protein 4 | ARL6IP4 | WHRSAGE | 332 | 0.0001 | 0.024 | 1.97 | Nucleus; DIA1 interactor |
| P0DMV9 | Heat shock 70 kDa protein 1B | HSPA1B | KGGSGSG | 631 | 0.0014 | 0.039 | 2.0 | Nucleus, cytoplasm, ER |
| Q8TB61 | Adenosine 3′-phospho 5′-phosphosulfate transporter 1 | SLC35B2 | PVESPVQ | 427 | 0.0004 | 0.024 | 1.9 | Golgi |
| Q8TDY2 | RB1-inducible coiled-coil protein 1 | RB1CC1 | RLDSLPE | 222 | 0.0022 | 0.046 | 1.7 | Cytoplasm, ER |
| P33981 | Dual specificity protein kinase TTK | TTK | LLNSPDC | 281 | 0.0008 | 0.039 | 1.6 | Cytoskeleton |
| Q9H307 | Pinin | PNN | RQESDPE | 100 | 0.0003 | 0.048 | 1.6 | Nucleus |
| Q8WVM8 | Sec1 family domain-containing protein 1 | SCFD1 | VENSPAG | 303 | 0.0212 | 0.156 | 1.5 | ER/Golgi |
| P27824 | Calnexin | CANX | GTVSQEE | 564 | 0.0310 | 0.179 | 1.49 | ER |
| P53621 | Coatomer subunit alpha | COPA | KNLSPGA | 173 | 0.0037 | 0.058 | 1.5 | Golgi |
| Q9NRX5 | Serine incorporator 1 | SERINC1 | SDGSLED | 364 | 0.0012 | 0.042 | 1.4 | ER |
| Q14004 | Cyclin-dependent kinase 13 | CDK13 | GDVSPSP | 383 | 0.0011 | 0.043 | 0.8 | Nucleus, cytoplasm, Golgi |
| Q56NI9 | N-acetyltransferase ESCO2 | ESCO2 | NQGSPFK | 75 | 0.0010 | 0.041 | 0.6 | Golgi, nucleus |
| O60238 | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like | BNIP3L | SSQSEEE | 120 | 0.0021 | 0.046 | 0.53 | Nucleus, ER, mitochondrion |
| Q9UQ35 | Serine/arginine repetitive matrix protein 2 | SRRM2 | LSYSPVE | 2694 | 0.0004 | 0.032 | 0.5 | Nucleus; DIA1 interactor |
| P67809 | Nuclease-sensitive element-binding protein 1 | YBX1 | NEGSESA | 174 | NA | NA | DOWN | Nucleus, cytosol; DIA1 interactor |
Notes:
All these phosphosites (except the one in TNNI3K) are listed in the PhosphositePlus database. For comparative analysis of all phosphosites, see the Supplemental Information. Note that for some phosphosites the t-test was not feasible due to their not being detected in one of the compared conditions (DIA1 or WT). Phosphosites whose difference between DIA1 and WT was not strictly significant are shown in italics. Full results are provided as Supplemental Information.