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Affinity precipitations of DnaK with C-terminal deletions of SStp. A, Amino acid sequences of His-S-SStp and C-terminal deletions. Residues in bold correspond to SStp. Black boxes indicate the N- and C-terminal His-tags, while gray boxes indicate N-terminal S-tags. B, Top, Far-western analysis of His-S-SStp deletions and the His-S tag alone using the S-protein conjugated to alkaline phosphatase; bottom, western blot of the samples in the top using α-DnaK antiserum.
