Procedure used to test the impact of negative mood induction on alcohol choice. At baseline, alcohol-seeking was measured by preference to select for enlargement alcohol versus food-related thumbnail images in two-alternative forced-choice trials. Negative mood was then induced by depressive statements and sad music (Samuel Barber’s Adagio for Strings), before alcohol-seeking was tested again in the same way. Subjective reactivity was indexed by the increase in sadness pre- and post-mood induction. The key question was whether the increase in percentage choice of alcohol versus food images from baseline to test (mood-induced alcohol-seeking) would be associated with depression symptoms, drinking to cope, alcohol use disorder symptoms, and subjective reactivity. ITI = intertrial interval. See the online article for the color version of this figure.