Figure 4.

Pre-B-cell leukemia transcription factor 3 (PBX3) is a direct target of miR-495 in melanoma cells. (A) Human PBX3 3′UTR binding site for miR-495. (B) miR-495 targeted the wild-type but not the mutant 3′UTR of PBX3. (C) Overexpression of miR-495 repressed PBX3 mRNA expression level (left) and protein level (middle and right) in A375 and MeWo cells. Data are presented as mean ± SD (n=3). All experiments were performed three times, and representative images are presented. **P<0.01.

Abbreviations: mut, mutant; NC, negative control; wt, wild-type.