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. 2018 Apr 5;11:1909–1920. doi: 10.2147/OTT.S152362

Figure 5.

Figure 5

Figure 5

Silencing of pre-B-cell leukemia transcription factor 3 (PBX3)-inhibited cell proliferation, migration, and invasion in melanoma cells. (A) A375 and MeWo cells were transfected with negative control siRNA (si-NC) or siRNA against PBX3 (si-PBX3) for 24 hours, the mRNA expression levels of PBX3 in both the cells were examined by RT-qPCR and (B) the protein levels of PBX3 in both the cells were examined by Western blot. (C) A375 and MeWo cells were transfected with si-NC or si-PBX3 for indicated time, and cell proliferation was analyzed by MTT assay. (D) A375 and MeWo cells were transfected with si-NC or by transwell assay. (E) Cell migration was analyzed by si-PBX3 for 24 hours, and cell invasion was analyzed by wound healing assay. Data are presented as mean ± SD (n=3). All experiments were performed three times, and representative images are presented. *P<0.05, **P<0.01, ***P<0.001.

Abbreviation: NC, negative control.