Table I.
Compilation of primers used
| Primer Name | Amino Acid Sequence | Nucleotide Sequencea | Restriction Site |
|---|---|---|---|
| 1Fb | DNPSNAc | GCGGAATTCGAYAAYCCIWSIAAYGC | EcoRI |
| 1Rb | FNV/LPHVAc | GCGGATCCGCIACRTGIGGIAHRTTRAA | BamHI |
| 2F | SNAVEWc | GCGGAATTCWSIAAYGCIRTIGARTGG | EcoRI |
| 2R | HPVAXFNc | GCGGATCCRTTRAAIIINGCIACIGGRTG | BamHI |
| 3F | d | GCGGAATTCCACACAGGAAACAGCTATGAC | EcoRI |
| 3Re | VVTRYSS | GCGGATCCAGACGAGTAGCGAGTCACAAC | BamHI |
| 4R#1f | TVLFLL | GCGGATCCAAGAGGAACAGTACT | BamHI |
| 4R#2f | ATLFLL | GCGGATCCAAGAGGAACAATGTG | BamHI |
| 5F#1f | g | GCGAATGCATTGCTCCCACTAGCC | |
| 5R#1f | MELITI | GCGATGGTTATGAGTTCCATTTTG | |
| 6F#1 (na) | MELITIL | GCGCATATGGAACTAATAACAATTCTT | NdeI |
| 6R | LLQSF*h | GCGAAGCTTATTAGAAGCTCTGGAGCAG | HindIII |
| 6F#1 [Δ(1–31)17α(8aa)] | MALLLAVFFLFLFKQ | GCGCATATGGCTCTGTTATTAGCAGTTTTTTTCC | |
| TCTTCCTCTTCAAACAA | NdeI | ||
| 6F#1 [Δ(1–52)2E1(10aa)] | MARQVHSSWNLPPGP | GCGCATATGGCTCGTCAAGTTCATTCTTCTTGG | |
| AATTTACCACCAGGCCCC | NdeI |
a
The sequence is shown from 5′ end to 3′ end.
b
F, Forward primer; R, reverse primer.
c
Amino acid consensus sequence based on alignment in Fig. 2 and used for primer design.
d
A specific primer for pcDNA2.1 placed just upstream the insertion site of the 5′ end of the cDNA library.
e
Covers a sequence that is identical in the two clones #1 and #2.
f
Covers a sequence that is specific for either of the two clones #1 and #2.
g
A specific primer for the 5′-UTR in #1.
h
The asterisk indicates a stop codon.