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. 2017 Apr 13;17(3):207–218. doi: 10.3727/105221617X695438

Figure 2.

Figure 2

Effect of a KB2115-supplemented diet on the S phase and mitotic activity of hepatocytes. Rats were fed a KB2115 diet (1.6 or 0.8 mg/kg of diet) for 1 week. (A) Representative microphotograph showing the liver of controls and KB2115-fed rats [arrows indicate the presence of mitoses in the liver of drug-fed animals; hematoxylin–eosin (H&E): 20×]. (B) Microphotograph illustrating the presence of several BrdU+ hepatocyte nuclei in the liver of KB2115-fed rats (0.8 mg/kg of diet) [sections stained with anti-BrdU antibody and counterstained with hematoxylin (20×)]. (C) LI and (D) mitotic index (MI). To label hepatocytes, animals received BrdU (1 g/L) in drinking water all throughout the treatment period. LI was expressed as the number of BrdU+ hepatocyte nuclei/100 nuclei. MI was calculated as the number of mitotic figures/1,000 nuclei. Results were expressed as means ± SE of three to five rats per group. At least 3,000 hepatocytes per liver were scored. *p < 0.05; ***p < 0.001.