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. 2017 Apr 13;17(3):207–218. doi: 10.3727/105221617X695438

Figure 4.

Figure 4

Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis of the expression of immediate early genes, Ccnd1, and TRβ target genes. Animals treated with a single IG dose of KB2115 (12.5 μg/100 g b.w.) or DMSO (5% in corn oil, Co) were sacrificed 1, 3, or 6 h after treatment. qRT-PCR analysis was performed also in the liver of rats subjected to two-thirds PH or laparotomy (sham operation). Expression of (A) c-fos, c-jun, and c-myc; (B) Ccnd1; (C) deiodinase 1 (Dio1) and glucose-6-phosphatase (G6pc) in rats subjected to laparotomy, PH, vehicle (Co), or KB2115 and sacrificed at the indicated times. Each sample was run in triplicate, and gene expression analysis of GAPDH was used as endogenous control. Relative quantification analysis for each gene was calculated by the 2−ΔΔCt method. *p < 0.05; **p < 0.01; ***p < 0.001.