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. 2017 Apr 13;17(3):207–218. doi: 10.3727/105221617X695438

Figure 6.

Figure 6

qRT-PCR analysis of the expression of β-catenin (Ctnnb1) and of its target genes (Axin2, Lgr5, and Glul). Expression of β-catenin, Axin2, Lgr5, and Glul in rats treated with (A) a single IG dose of KB2115 (12.5 μg/100 g b.w.) or DMSO (5% in corn oil, Co) and sacrificed at 1, 3, or 6 h or (B) fed a KB2115-supplemented diet (0.8 or 1.6 mg/kg) and sacrificed 1 week later. Each sample was run in triplicate, and gene expression analysis of GAPDH was used as endogenous control. Relative quantification analysis for each gene was calculated by the 2−ΔΔCt method. *p < 0.05; **p < 0.01.